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| Chitosan Biomaterials |
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Chitosan is an abundant natural polysaccharide that has several medical applications. It is a polymer of glucosamine that is partly acetylated and can be biodegraded in vivo. We have developed particular formulations of chitosan that can be used in conjunction with blood, cells and other biological components in order to repair tissues and have other therapeutic effects. Our research in chitosan is both fundamental and applied. In the fundamental area we are studying the polyelectrolyte behavior of chitosan in solution and in particular examining its interesting thermal gelation upon heating. The molecular mechanisms involved in gelation and other physicochemical phenomena are being investigated both experimentally and with theoretical mathematical models of polyelectrolytes in solution. The physical and biological properties of chitosan are very dependent on its molecular weight and distribution of acetyl groups. Therefore our research efforts also involve development of processes to control molecular weight and acetylation and the development of analytical methods to precisely characterize these two parameters. |
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| Scientific Articles |
Bell, A.D.,
Lascau-Coman, V.,
Sun,J.,
Chen, G.,
Lowerison, M. W.,
Hurtig, M.B.
Hoemann, C.D. (2013). "Bone-Induced Chondroinduction in Sheep Jamshidi Biopsy Defects with and without Treatment by Subchondral Chitosan-Blood Implant 1-Day, 3-Week, and 3-Month Repair" Cartilage OnlineFirst (October 2012): 1-12. PDF
| Hoemann, C. D.,
Guzman-Morales, J.,
Tran-Khanh, N.,
Lavallee, G.,
Jolicoeur, M.,
Lavertu, M., (2013). "Chitosan Rate of Uptake in HEK293 Cells is Influenced by Soluble versus Microparticle State and Enhanced by Serum-Induced Cell Metabolism and Lactate-Based Media Acidification" Molecules 18(1): 1015-1035. PDF
| Lavertu M., Darras V. and Buschman M. D. (2012). "Kinetics and efficiency of chitosan reacetylation" Carbohydrate Polymers 87(2): 1192-1198. PDF
| Alameh M., DeJesus D., Jean M., Darras V., Thibault M., Lavertu M., Buschmann M. D. and Merzouki A. (2012). "Low molecular weight chitosan nanoparticulate system at low N:P ratio for nontoxic polynucleotide delivery" International Journal of Nanomedecine 7 1399-1414. PDF
| Marchand C., Chen G., Tran-Khanh N., Sun J., Chen H., Buschmann M. D. and Hoemann C. D. (2011). "Microdrilled Cartilage Defects Treated with Thrombin-Solidified Chitosan/Blood Implant Regenerate a More Hyaline, Stable, and Structurally Integrated Osteochondral Unit Compared to Drilled Controls" Tissue Engineering Part A Epub 11 NOV 2011 1-12. PDF
| Jean M., Alameh M., Buschmann M. D. and Merzouki A. (2011). "Effective and safe gene-based delivery of GLP-1 using chitosan/plasmid-DNA therapeutic nanocomplexes in an animal model of type 2 diabetes" Gene Therapy 18(8): 807-816. PDF
| Guzman-Morales J., Ariganello M. B., Hammami I., Thibault M., Jolicoeur M. and Hoemann C. D. (2011). "Biodegradable chitosan particles induce chemokine release and negligible arginase-1 activity compared to IL-4 in murine bone marrow-derived macrophages" Biochemical and Biophysical Research Communications 405(4): 538-544. PDF
| Thibault M., Astolfi M., Tran-Khanh N., Lavertu M., Darras V., Merzouki A. and Buschmann M. D. (2011). "Excess polycation mediates efficient chitosan-based gene transfer by promoting lysosomal release of the polyplexes" Biomaterials 32(Epub ahead of print): 4639-4646. PDF
| Chen G., Sun J., Lascau-Coman V., Chevrier A., Marchand C. and Hoemann C. D. (2011). "Acute osteoclast activity following subchondral drilling is promoted by chitosan and associated with improved cartilage repair tissue integration" Cartilage 2(2): 173-185. PDF
| Hoemann C. D., Chen G. P., Marchand C., Tran-Khanh N., Thibault M., Chevrier A., Sun J., Shive M. S., Fernandes M. J. G., Poubelle P. E., Centola M. and El-Gabalawy H. (2010). " Scaffold-guided subchondral bone repair: Implication of neutrophils and alternatively activated Arginase-1+Macrophages" American Journal of Sports Medicine 38(9): 1845-1856. PDF
| Marchand C., Bachand J., Perinet J., Baraghis E., Lamarre M., Rivard G. E., De Crescenzo G. and Hoemann C. D. (2010). " C3, C5, and factor B bind to chitosan without complement activation" Journal of Biomedical Materials Research Part A 93A(4): 1429-1441. PDF
| Darras V., Nelea M., Winnik F. M. and Buschmann M. D. (2010). " Chitosan modified with gadolinium diethylenetriaminepentaacetic acid for magnetic resonance imaging of DNA/chitosan nanoparticles" Carbohydrate Polymers 80(4): 1137-1146. PDF
| Ma P. L., Buschmann M. D. and Winnik F. M. (2010). " Complete physicochemical characterization of DNA/Chitosan complexes by multiple detection using asymmetrical Flow Field-Flow Fractionation" Analytical Chemistry 82(23): 9636-9643. PDF
| Alameh M., Jean M., DeJesus D., Buschmann M. D. and Merzouki A. (2010). "Chitosanase-based method for RNA isolation from cells transfected with chitosan/siRNA nanocomplexes for real-time RT-PCR in gene silencing" International Journal of Nanomedicine 5 473-481. PDF
| Ma P. L., Buschmann M. D. and Winnik F. M. (2010). "One-Step Analysis of DNA/Chitosan Complexes by Field-Flow Fractionation Reveals Particle Size and Free Chitosan Content" Biomacromolecules 11(3): 549-554. PDF
| Tran-Khanh N., Chevrier A., Lascau-Coman V., Hoemann C. D. and Buschmann M. D. (2010). " Young Adult Chondrocytes Proliferate Rapidly and Produce a Cartilaginous Tissue at the Gel-Media Interface in Agarose Cultures" Connective Tissue Research 51(3): 216-223. PDF
| Simard P., Galarneau H., Marois S., Rusu D., Hoemann C. D., Poubelle P. E., El-Gabalawy H. and Fernandes M. J. G. (2009). " Neutrophils exhibit distinct phenotypes toward chitosans with different degrees of deacetylation: implications for cartilage repair" Arthritis Research & Therapy 11(3): 1-10. PDF
| Guzman-Morales J., El-Gabalawy H., Pham M. H., Tran-Khanh N., McKee M. D., Wu W., Centola M. and Hoemann C. D. (2009). " Effect of chitosan particles and dexamethasone on human bone marrow stromal cell osteogenesis and angiogenic factor secretion" Bone 45(4): 617-626. PDF
| Nguyen S., Hisiger S., Jolicoeur M., Winnik F. M. and Buschmann M. D. (2009). " Fractionation and characterization of chitosan by analytical SEC and H-1 NMR after semi-preparative SEC" Carbohydrate Polymers 75(4): 636-645. PDF
| Nguyen S., Winnik F. M. and Buschmann M. D. (2009). " Improved reproducibility in the determination of the molecular weight of chitosan by analytical size exclusion chromatography" Carbohydrate Polymers 75(3): 528-533. PDF
| Jean M., Smaoui F., Lavertu M., Methot S., Bouhdoud L., Buschmann M. D. and Merzouki A. (2009). " Chitosan-plasmid nanoparticle formulations for IM and SC delivery of recombinant FGF-2 and PDGF-BB or generation of antibodies" Gene Therapy 16(9): 1097-1110. PDF
| Ma P. L., Lavertu M., Winnik F. M. and Buschmann M. D. (2009). " New insights into chitosan-DNA interactions using isothermal titration microcalorimetry" Biomacromolecules 10(6): 1490-1499. PDF
| Ma O., Lavertu M., Sun J. , Nguyen S., Buschmann MD. , Winnik F. and Hoemann CD. (2008). " Precise derivatization of structurally distinct chitosans with rhodamine B isothiocyanate" Carbohydrate polymers 72(4): 616-624. PDF
| Iliescu M., Hoemann C. D., Shive M. S., Chenite A. and Buschmann M. D. (2008). " Ultrastructure of hybrid chitosan-glycerol phosphate blood clots by environmental scanning electron Microscopy" Microscopy Research and Technique 71(3): 236-247. PDF
| Demolliens A., Boucher C., Durocher Y., Jolicoeur M., Buschmann M. D. and De Crescenzo G. (2008). " Tyrosinase-catalyzed synthesis of a universal coil-chitosan bioconjugate for protein immobilization" Bioconjugate Chemistry 19(9): 1849-1854. PDF
| Lavertu M., Filion D. and Buschmann M. D. (2008). " Heat-induced transfer of protons from chitosan to glycerol phosphate produces chitosan precipitation and gelation" Biomacromolecules 9(2): 640-650. PDF
| Hoemann C. D., Sun J., McKee M. D., Chevrier A., Rossomacha E., Rivard G. E., Hurtig M. and Buschmann M. D. (2007). " Chitosan-glycerol phosphate/blood implants elicit hyaline cartilage repair integrated with porous subchondral bone in microdrilled rabbit defects" Osteoarthritis and Cartilage 15(1): 78-89. PDF
| Filion D., Lavertu M. and Buschmann M.D. (2007). " Ionization and solubility of chitosan solutions related to thermosensitive chitosan/glycerol-phosphate systems" Biomacromolecules 8(NA): 3224-3234. PDF
| Hoemann C. D., Chenite A., Sun J., Hurtig M., Serreqi A., Lu Z., Rossomacha E. and Buschmann M. D. (2007). " Cytocompatible gel formation of chitosan-glycerol phosphate solutions supplemented with hydroxyl ethyl cellulose is due to the presence of glyoxal" Journal of Biomedical Materials Research Part A 83A(2): 521-529. PDF
| Chenite A., Gori S., Shive M. and Desrosiers E. Buschmann M. D. (2006). " Monolithic gelation of chitosan solution via enzymatic hydrolysis of orea" Carbohydrate Polymers 64(NA): 419-424. PDF
| Chevrier A., Hoemann C. D., Sun J. and Buschmann M. D. (2006). " Chitosan-glycerol phosphate/blood implants increase cell recruitment, transient vascularization and subchondral bone remodeling in drilled cartilage defects" Osteoarthritis and Cartilage 15(3): 316-327. PDF
| Hoemann C.D. , Hurtig M, Rossomacha E., Sun J, Chevrier A. , Shive MS. and Buschmann MD. (2005). " Chitosan-glycerol phosphate/blood implants improve hyaline cartilage repair in ovine microfracture defects" J Bone Joint Surg Am. 87(12): 2671-2686. PDF
| Gauthier M. A., Luo J., Calvet D., Ni C., Zhu X. X., Garon M. and Buschmann M. D. (2004). " Degree of crosslinking and mechanical properties of crosslinked poly(vinyl alcohol) beads for use in solid-phase organic synthesis" Polymer 45(NA): 8201-8210. PDF
| Lavertu M., Xia Z., Serreqi A. N., Berrada M., Rodrigues A., Wang D., Buschmann M. D. and Gupta A. (2003). " A validated 1H NMR method for the determination of the degree of deacetylation of chitosan" J Pharm Biomed Anal 32(6): 1149-58. PDF
| Hoemann C. D., Sun J., Chrzanowski V. and Buschmann M. D. (2002). " A multivalent assay to detect glycosaminoglycan, protein, collagen, RNA, and DNA content in milligram samples of cartilage or hydrogel- based repair cartilage" Anal Biochem 300(1): 1-10. PDF
| Jarry C., Chaput C., Chenite A., Renaud M. A., Buschmann M. and Leroux J. C. (2001). " Effects of steam sterilization on thermogelling chitosan-based gels" J. Biomed Mater Res 58(1): 127-135. PDF
| Chenite A., Buschmann M., Wang D., Chaput C. and Kandani N. (2001). "Rheological characterisation of thermogelling chitosan/glycerol-phosphate solutions" Carbohydrate Polymers 46(1): 39-47. PDF
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Cartilage and Meniscus Repair |
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The knee joint and other joints in the body are organs composed of tissues such as cartilage, meniscus, ligaments, tendons and bones that must all function together. Unfortunately traumatic injury to these tissues and other disease processes can damage them to the point of limiting joint motion and causing pain, ultimately leading to arthritis and dysfunction. We are studying the structure, biomechanics and biology of articular cartilage and meniscus and using chitosan based biomaterials to develop means of regenerating these tissues when they are damaged. Our approach relies on formulating mixtures of chitosan solutions and blood components that can be applied to cartilage and meniscus lesions and stimulate their repair by activating host cell recruitment, vascularisation and synthesis of an effective repair tissue. We have succeeded in improving the repair of articular cartilage in animal models and this technology has been translated to industry for clinical application.
(http://www.biosyntech.com/en/expertise/orthopedics/?BST=CarGel)
The formulations are being refined and extended for use in other contexts and for the repair of other tissues. |
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| Scientific Articles |
Hoemann, C.D.,
Gosselin, Y.,
Chen, H.,
Sun, J.,
Hurtig, M.B.,
Carli, A.,
Stanish, W. D. (2013). "Characterization of Initial Microfracture Defects in Human Condyles" The journal of knee surgery epub ahead of print(:): 1-10. PDF
| Bell, A.D.,
Lascau-Coman, V.,
Sun,J.,
Chen, G.,
Lowerison, M. W.,
Hurtig, M.B.
Hoemann, C.D. (2013). "Bone-Induced Chondroinduction in Sheep Jamshidi Biopsy Defects with and without Treatment by Subchondral Chitosan-Blood Implant 1-Day, 3-Week, and 3-Month Repair" Cartilage OnlineFirst (October 2012): 1-12. PDF
| Lafantaisie-Favreau, C.-H.,
Guzmán-Morales, J.,
Sun, J.,
Chen, G.,
Harris, A.,
Smith, T. D.,
Carli, A.,
Henderson, J.,
Stanish,W.D.,
Hoemann, C. D. (2013). "Subchondral pre-solidified chitosan/blood implants elicit reproducible early osteochondral wound-repair responses including neutrophil and stromal cell chemotaxis, bone resorption and repair, enhanced repair tissue integration and delayed matrix depo" BMC Musculoskeletal Disorders 14(27): 1-16. PDF
| Hoemann C. D.,
Lafantaisie-Favreau C. H.,
Lascau-Coman V.,
Chen G.
Guzmán-Morales J. (2012). "The cartilage-bone interface" The Journal of Knee Surgery 25(2): 85-97. PDF
| Chen H., Hoemann C. D., Sun J., Chevrier A., McKee M. D., Shive M. S., Hurtig M. and Buschmann M. D. (2011). "Depth of subchondral perforation influences the outcome of bone marrow stimulation cartilage repair" Journal of Orthopaedic Research On-line(24-FEB-2011): 1-14. PDF
| Changoor A., Nelea M., Méthot S., Tran-Khanh N., Chevrier A., Restrepo A., Shive M. S., Hoemann C. D. and Buschmann M. D. (2011). "Structural characteristics of the collagen network in human normal, degraded and repair articular cartilages observed in polarized light and scanning electron microscopies" Osteoarthritis Cartilage 19(12): 1458-1468. PDF
| Changoor A., Coutu J. P., Garon M., Quenneville E., Hurtig M. B. and Buschman M. D. (2011). "Streaming potential-based arthroscopic device is sensitive to cartilage changes immediately post-impact in an equine cartilage injury model" Journal of Biomechanical Engineering-Transactions of the Asme 133(6): 1-10. PDF
| Guzman-Morales J., Ariganello M. B., Hammami I., Thibault M., Jolicoeur M. and Hoemann C. D. (2011). "Biodegradable chitosan particles induce chemokine release and negligible arginase-1 activity compared to IL-4 in murine bone marrow-derived macrophages" Biochemical and Biophysical Research Communications 405(4): 538-544. PDF
| Hoemann C., Kandel R., Roberts S., Saris D. B. F., Creemers L. B., Mainil-Varlet P., Methot S., Hollander A. P. and Buschman M. D. (2011). "International Cartilage Repair Society (ICRS) Recommended Guidelines for Histological Endpoints for Cartilage Repair Studies in Animal Models and Clinical Trials " Cartilage 2(2): 153-172. PDF
| Changoor A., Tran-Khanh N., Methot S., Garon M., Hurtig M. B., Shive M. S. and Buschmann M. D. (2011). "A polarized light microscopy method for accurate and reliable grading of collagen organization in cartilage repair" Osteoarthritis and Cartilage 19(1): 126-135. PDF
| Chen G., Sun J., Lascau-Coman V., Chevrier A., Marchand C. and Hoemann C. D. (2011). "Acute osteoclast activity following subchondral drilling is promoted by chitosan and associated with improved cartilage repair tissue integration" Cartilage 2(2): 173-185. PDF
| Marchand C., Chen H., Buschmann M. D. and Hoemann C. D. (2011). "Standardized 3D volumes of interest with adapted surfaces for more precise subchondral bone analyses by micro-CT" Tissue Engineering Part C-Methods 17(4): 475-484. PDF
| Hurtig M., Buschman M. D., Fortier L. A., Hoemann C. D., Hunziker E., Jurvelin J., Mainil-Varlet P., McIlwraith C. W., Sah R. L. and Whiteside R. (2011). "Preclinical studies for cartilage repair: recommendations from the Internatinoal Cartilage Repair Society" Cartilage 2(2): 137-152. PDF
| Kazemi M., Li L. P., Savard P. and Buschmann M. D. (2011). "Creep behavior of the intact and meniscectomy knee joints" Journal of the Mechanical Behavior of Biomedical Materials 4(7): 1351-1358. PDF
| Chen H. M., Chevrier A., Hoemann C. D., Sun J., Ouyang W. and Buschmann M. D. (2011). "Characterization of Subchondral Bone Repair for Marrow-Stimulated Chondral Defects and Its Relationship to Articular Cartilage Resurfacing" American Journal of Sports Medicine 39(8): 1731-1740. PDF
| Marchand C., Chen G., Tran-Khanh N., Sun J., Chen H., Buschmann M. D. and Hoemann C. D. (2011). "Microdrilled Cartilage Defects Treated with Thrombin-Solidified Chitosan/Blood Implant Regenerate a More Hyaline, Stable, and Structurally Integrated Osteochondral Unit Compared to Drilled Controls" Tissue Engineering Part A Epub 11 NOV 2011 1-12. PDF
| Kazemi M., Li L. P., Buschman M. D. and Savard P. (2011). "Partial meniscetomy changes fluid pressurization in articular cartilage in human knees" Journal of Biomechanical Engineering In press . PDF
| El-Bikai R., Welman M., Margaron Y., Cote J. F., Macqueen L., Buschmann M. D., Fahmi H., Shi Q., Maghni K., Fernandes J. C. and Benderdour M. (2010). " Perturbation of adhesion molecule-mediated chondrocyte-matrix interactions by 4-hydroxynonenal binding: implication in osteoarthritis pathogenesis" Arthritis Research & Therapy 12(5): 1-14. PDF
| Chevrier A., Hoemann C. D., Sun J. and Buschmann M. D. (2010). "Temporal and spatial modulation of chondrogenic foci in subchondral microdrill holes by chitosan-glycerol phosphate/blood implant" Osteoarthritis and cartilage Epub ahead of print(xxx): 1-9. PDF
| Poole R., Blake S., Buschmann M., Goldring S., Laverty S., Lockwood S., Matyas J., McDougall J., Pritzker K., Rudolphi K., van den Berg W. and Yaksh T. (2010). "Recommendations for the use of preclinical models in the study and treatment of osteoarthritis" Osteoarthritis and Cartilage 18 S10-S16. PDF
| Marchand C., Bachand J., Perinet J., Baraghis E., Lamarre M., Rivard G. E., De Crescenzo G. and Hoemann C. D. (2010). " C3, C5, and factor B bind to chitosan without complement activation" Journal of Biomedical Materials Research Part A 93A(4): 1429-1441. PDF
| Tran-Khanh N., Chevrier A., Lascau-Coman V., Hoemann C. D. and Buschmann M. D. (2010). " Young Adult Chondrocytes Proliferate Rapidly and Produce a Cartilaginous Tissue at the Gel-Media Interface in Agarose Cultures" Connective Tissue Research 51(3): 216-223. PDF
| Hoemann C. D., Chen G. P., Marchand C., Tran-Khanh N., Thibault M., Chevrier A., Sun J., Shive M. S., Fernandes M. J. G., Poubelle P. E., Centola M. and El-Gabalawy H. (2010). " Scaffold-guided subchondral bone repair: Implication of neutrophils and alternatively activated Arginase-1+Macrophages" American Journal of Sports Medicine 38(9): 1845-1856. PDF
| Guzman-Morales J., El-Gabalawy H., Pham M. H., Tran-Khanh N., McKee M. D., Wu W., Centola M. and Hoemann C. D. (2009). " Effect of chitosan particles and dexamethasone on human bone marrow stromal cell osteogenesis and angiogenic factor secretion" Bone 45(4): 617-626. PDF
| Hoemann C. D., El-Gabalawy H. and McKee M. D. (2009). " In vitro osteogenesis assays: Influence of the primary cell source on alkaline phosphatase activity and mineralization" Pathologie Biologie 57(4): 318-323. PDF
| Simard P., Galarneau H., Marois S., Rusu D., Hoemann C. D., Poubelle P. E., El-Gabalawy H. and Fernandes M. J. G. (2009). " Neutrophils exhibit distinct phenotypes toward chitosans with different degrees of deacetylation: implications for cartilage repair" Arthritis Research & Therapy 11(3): 1-10. PDF
| Marchand C. , Rivard G. E., Sun J. and Hoemann C. D. (2009). " Solidification mechanisms of chitosan-glycerol phosphate/blood implant for articular cartilage repair" Osteoarthritis and Cartilage 17(7): 953-960. PDF
| Gigout A., Levasseur S., Girard-Lauriault P. L., Buschmann M. D., Wertheimer M. R. and Jolicoeur M. (2009). " CHO cells adhering to nitrogen-rich plasma-polymerised ethylene exhibit high production of a specific recombinant protein" Macromolecular Bioscience 9(10): 979-988. PDF
| Gigout A., Buschmann M. D. and Jolicoeur M. (2009). " Chondrocytes cultured in stirred suspension with serum-free medium containing pluronic-68 aggregate and proliferate while maintaining their differentiated phenotype." Tissue Engineering Part A 15(8): 2237-2248. PDF
| Chevrier A., Nelea M., Hurtig M. B., Hoemann C. D. and Buschmann M. D. (2009). " Meniscus structure in human, sheep, and rabbit for animal models of meniscus repair" Journal of Orthopaedic Research 27(9): 1197-1203. PDF
| Chen H., Sun J., Hoemann C.D, Lascau-Coman V., Ouyang W., McKee M. D., Shive M.S. and Buschmann M.D. (2009). " Drilling and microfracture lead to different bone structure and necrosis during bone-marrow stimulation for cartilage repair" Journal of Orthopaedic Research 27(11): 1432-1438. PDF
| Gigout A., Buschmann M. D. and Jolicoeur M. (2008). " The fate of Pluronic F-68 in chondrocytes and CHO cells" Biotechnology and Bioengineering 100(5): 975-987. PDF
| Gigout A., Jolicoeur M., Nelea M., Raynal N., Farndale R. and Buschmann M. D. (2008). " Chondrocyte aggregation in suspension culture is GFOGER-GPP- and beta 1 Integrin-dependent" Journal of Biological Chemistry 283(46): 31522-31530. PDF
| Hoemann C. D., Sun J., McKee M. D., Chevrier A., Rossomacha E., Rivard G. E., Hurtig M. and Buschmann M. D. (2007). " Chitosan-glycerol phosphate/blood implants elicit hyaline cartilage repair integrated with porous subchondral bone in microdrilled rabbit defects" Osteoarthritis and Cartilage 15(1): 78-89. PDF
| Thibault M. M., Hoemann C. D. and Buschmann M. D. (2007). "Fibronectin, vitronectin, and collagen I induce chemotaxis and haptotaxis of human and rabbit mesenchymal stem cells in a standardized transmembrane assay" Stem Cells and Development 16(3): 489-502. PDF
| Thibault M. M. and Buschmann M. D. (2006). " Migration of bone marrow stromal cells in 3D: 4 color methodology reveals spatially and temporally coordinated events" Cell Motility and the Cytoskeleton 63(12): 725-740. PDF
| Valverde-Franco G., Binette J. S., Li W., Wang H., Chai S., Laflamme F., Tran-Khanh N., Quenneville E., Meijers T., Poole A. R., Mort J. S., Buschmann M. D. and Henderson J. E. (2006). " Defects in articular cartilage metabolism and early arthritis in fibroblast growth factor receptor 3 deficient mice" Hum Mol Genet 15(11): 1783-92. PDF
| Chevrier A., Hoemann C. D., Sun J. and Buschmann M. D. (2006). " Chitosan-glycerol phosphate/blood implants increase cell recruitment, transient vascularization and subchondral bone remodeling in drilled cartilage defects" Osteoarthritis and Cartilage 15(3): 316-327. PDF
| Shive M. S., Hoemann C. D., Restrepo A., Hurtig M. B., Duval N., Ranger P., Stanish W. and Buschmann M. D. (2006). " BST-Cargel: in situ chondroInduction for cartilage repair" Operative Techniques in Orthopaedics 16(NA): 271-278. PDF
| Davidson D., Blanc A., Filion D., Wang H., Plut P., Pfeffer G., Buschmann M. D. and Henderson J. E. (2005). " Fibroblast growth factor (FGF) 18 signals through FGF receptor 3 to promote chondrogenesis" J Biol Chem 280(21): 20509-15. PDF
| Hoemann C.D. , Hurtig M, Rossomacha E., Sun J, Chevrier A. , Shive MS. and Buschmann MD. (2005). " Chitosan-glycerol phosphate/blood implants improve hyaline cartilage repair in ovine microfracture defects" J Bone Joint Surg Am. 87(12): 2671-2686. PDF
| Blanc A., Tran-Khanh N., Filion D. and Buschmann M. D. (2005). " Optimal processing method to obtain four-color confocal fluorescent images of the cytoskeleton and nucleus in three-dimensional chondrocyte cultures" Journal of Histochemistry & Cytochemistry 53(9): 1171-1175. PDF
| Chevrier A., Rossomacha E., Buschmann M. D. and Hoemann C. D. (2005). " Optimization of histoprocessing methods to detect glycosaminoglycan, collagen type II, and collagen type I in decalcified rabbit osteochondral sections" Journal of Histotechnology 28(3): 165-175. PDF
| Quenneville Eric and Buschmann Michael D. (2005). " A transport model of electrolyte convection through a charged membrane predicts generation of net charge at membrane/electrolyte inerfaces" Journal of Membrane Science 265(NA): 60-73. PDF
| Gigout A., Jolicoeur M. and Buschmann M. D. (2005). " Low calcium levels in serum-free media maintain chondrocyte phenotype in monolayer culture and reduce chondrocyte aggregation in suspension culture" Osteoarthritis Cartilage 13(11): 1012-24. PDF
| Hoemann C. D., Sun J., Legare A., McKee M. D. and Buschmann M. D. (2005). " Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell-delivery vehicle" Osteoarthritis Cartilage 13(4): 318-329. PDF
| Quenneville E., Binette J. S., Garon M., Legare A., Meunier M. and Buschmann M. D. (2004). " Fabrication and characterization of nonplanar microelectrode array circuits for use in arthroscopic diagnosis of cartilage diseases" IEEE Transactions on Biomedical Engineering 51(12): 2164-2173. PDF
| Binette J. S., Garon M., Savard P., McKee M. D. and Buschmann M. D. (2004). " Tetrapolar measurement of electrical conductivity and thickness of articular cartilage" Journal of Biomechanical Engineering 126(4): 475-484. PDF
| Li L. P., Buschmann M. D. and Shirazi-Adl A. (2003). " Strain-rate dependent stiffness of articular cartilage in unconfined compression" J Biomech Eng 125(2): 161-168. PDF
| Jurvelin J. S., Buschmann M. D. and Hunziker E. B. (2003). " Mechanical anisotropy of the human knee articular cartilage in compression" Proc Inst Mech Eng [H] 217(3): 215-219. PDF
| Fortin M., Buschmann M. D., Bertrand M. J., Foster F. S. and Ophir J. (2003). " Dynamic measurement of internal solid displacement in articular cartilage using ultrasound backscatter" Journal of Biomechanics 36(3): 443-447. PDF
| Li L. P., Shirazi-Adl A. and Buschmann M. D. (2003). " Investigation of mechanical behavior of articular cartilage by fibril reinforced poroelastic models" Biorheology 40(NA): 227-233. PDF
| Hoemann C. D., Sun J., Chrzanowski V. and Buschmann M. D. (2002). " A multivalent assay to detect glycosaminoglycan, protein, collagen, RNA, and DNA content in milligram samples of cartilage or hydrogel- based repair cartilage" Anal Biochem 300(1): 1-10. PDF
| Légaré A., Garon M., Guardo R., Savard P., Poole A. R. and Buschmann M. D. (2002). " Detection and analysis of cartilage degeneration by spatially resolved streaming potentials in articular cartilage" Journal of Orthopaedic Research 20(4): 819-826. PDF
| Garon M., Légaré A., Guardo R., Savard P. and Buschmann M. D. (2002). " Streaming potentials maps are spatially resolved indicators of amplitude, frequency and ionic strenght dependant responses of articular cartilage to load" Journal of Biomechanics 35(2): 207-216. PDF
| Ravenelle F., Marchessault R. H., Legare A. and Buschmann M. D. (2002). " Mechanical properties and structure of swollen crosslinked high amylose starch tablets" Carbohydrate Polymers 47(3): 259-266. PDF
| Li L. P., Buschmann M. D. and Shirazi-Adl A. (2002). " The role of fibril reinforcement in the mechanical behavior of cartilage" Biorheology 39(NA): 89-96. PDF
| Thibault M., Poole A. R. and Buschmann M. D. (2002). " Cyclic compression of cartilage/bone explants in vitro leads to physical weakening, mechanical breakdown of collagen and release of matrix fragments" Journal of Orthopaedic Research 20(6): 1265-1273. PDF
| Li L., Shirazi-Adl A. and Buschmann M. (2002). " Alterations in mechanical behavior of articular cartilage due to changes in depth varying material properties a nonhomogeneous poroelastic model study" Computer Methods in Biomechanics and Biomedical Engineering 5(1): 45-52. PDF
| Langelier Eve and Buschmann Michael D. (2002). " Increasing strain and strain-rate strengthens transient stiffness but weakens the response to subsequent compression for articular cartilage in unconfined compression" Journal of Biomechanics 36(6): 853-859. PDF
| Joiner G. A., Bogoch E. R., Pritzker K. P., Buschmann M. D., Chevrier A. and Foster F. S. (2001). " High frequency acoustic parameters of human and bovine articular cartilage following experimentally-induced matrix degradation" Ultrason Imaging 23(2): 106-16. PDF
| Chevrier A., Mort J. S., Crine P., Hoemann C. D. and Buschmann M. D. (2001). " Soluble recombinant neprilysin induces aggrecanase-mediated cleavage of aggrecan in cartilage explant cultures" Arch Biochem Biophys 396(2): 178-86. PDF
| Li L. P., Buschmann M. D. and Shirazi-Adl A. (2001). " The asymmetry of transient response in compression vs release for cartilage in unconfined compression" Journal of Biomechanics 123(NA): 519-522. PDF
| Li L. P., Buschmann M. D. and Shirazi-Adl A. (2000). " A fibril reinforced nonhomogeneous poroelastic model for articular cartilage: inhomogeneous response in unconfined compression" Journal of Biomechanics 33(12): 1533-1541. PDF
| Chenite A., Chaput C., Wang D., Combes C., Buschmann M. D., Hoemann C. D., Leroux J. C., Binette F. and Selmani A. (2000). " Novel injectable neutral solutions of chitosan form biodegradable gels in situ" Biomaterials 21(21): 2155-2161. PDF
| Fortin M., Soulhat J., Shirazi-Adl A., Hunziker E. B. and Buschmann M. D. (2000). " Unconfined compression of articular cartilage: nonlinear behavior and comparison with a fibril-reinforced biphasic model" Journal of Biomechanical Engineering 122(NA): 189-195. PDF
| Langelier E., Suetterlin R., Hoemann C. D., Aebi U. and Buschmann M. D. (2000). " The chondrocyte cytoskeleton in mature articular cartilage: structure and distribution of actin, tubulin, and vimentin filaments" J Histochem Cytochem 48(10): 1307-1320. PDF
| Buschmann M. D., Maurer A. M., Berger E. and Hunziker E. B. (2000). "Ruthenium hexaammine trichloride chemography for aggrecan mapping in cartilage is a sensitive indicator of matrix degradation" The Journal of Histochemistry & Cytochemistry 48(1): 81-88. PDF
| Li L. P., Soulhat J., Buschmann M. D. and Shirazi-Adl A. (1999). " Nonlinear analysis of cartilage in unconfined ramp compression using a fibril reinforced poroelastic model" Clinical Biomechanics 14(9): 673-682. PDF
| Dumont J., Ionescu M., Reiner A., Poole A. Robin, Tran-Khanh N., Hoemann C. D., McKee M. and Buschmann M. D. (1999). " Mature full-thickness articular cartilage explants attached to bone are physiologically stable over long-term culture in serum-free media" Connective Tissue Research 40(4): 259-272. PDF
| Buschmann M. D., Kim Y. J., Wong M., Frank E., Hunziker E. B. and Grodzinsky A. J. (1999). " Stimulation of aggrecan synthesis in cartilage explants by cyclic loading is localized to regions of high interstitial fluid flow" Archives of Biochemistry and Biophysics 366(1): 1-7. PDF
| Soulhat J., Buschmann M. D. and Shirazi-Adl A. (1999). " A fibril-network reinforced biphasic model of cartilage in unconfined compression" Journal of Biomechanical Engineering 121(3): 340-347. PDF
| Quinn T. M., Grodzinsky A. J., Buschmann M. D., Kim Y. J. and Hunziker E. B. (1998). " Mechanical compression alters proteoglycan deposition and matrix deformation around individual cells in cartilage explants" J Cell Sci 111(NA): 573-583. PDF
| Buschmann M. D., Soulhat J., Shirazi-Adl A., Jurvelin J. S. and Hunziker E. B. (1998). " Confined compression of articular cartilage: linearity in ramp and sinusoidal tests and the importance of interdigitation and incomplete confinement" Journal of Biomechanics 31(2): 171-178. PDF
| Buschmann M. D. (1997). " Numerical conversion of transient to harmonic response functions for linear viscoelastic materials" Journal of Biomechanics 30(2): 197-202. PDF
| Jurvelin J. S., Buschmann M. D. and Hunziker E. B. (1997). " Optical and mechanical determination of Poisson's ratio of adult bovine humeral articular cartilage" Journal of Biomechanics 30(3): 235-41. PDF
| Wong M., Wuethrich P., Buschmann M. D., Eggli P. and Hunziker E. (1997). " Chondrocyte biosynthesis correlates with local tissue strain in statically compressed adult articular cartilage" Journal of Orthopaedic Research 15(2): 189-96. PDF
| Buschmann M. D., Hunziker E. B., Kim Y. J. and Grodzinsky A. J. (1996). " Altered aggrecan synthesis correlates with cell and nucleus structure in statically compressed cartilage" Journal of Cell Science 109(NA): 499-508. PDF
| Buschmann M. D., Maurer A. M., Berger E. and Hunziker E. B. (1996). " A method of quantitative autoradiography for the spatial localization of proteoglycan synthesis rates in cartilage" Journal of Histochemistry & Cytochemistry 44(5): 423-431. PDF
| Studer D., Michel M., Wohlwend M., Hunziker E. B. and Buschmann M. D. (1995). " Vitrification of articular cartilage by high-pressure freezing" Journal of Microscopy 179(Part 3): 321-332. PDF
| Buschmann M. D. and Grodzinsky A. J. (1995). " A molecular model of proteoglycan-associated electrostatic forces in cartilage mechanics " Journal of Biomechanical Engineering-Transactions of the Asme 117(2): 179-192. PDF
| Buschmann M. D., Gluzband Y. A., Grodzinsky A. J. and Hunziker E. B. (1995). " Mechanical compression modulates matrix biosynthesis in chondrocyte agarose cultures" Journal of Cell Science 108(NA): 1497-1508. PDF
| Buschmann M. D., Gluzband Y. A., Grodzinsky A. J., Kimura J. H. and Hunziker E. B. (1992). " Chondrocytes in agarose culture synthesize a mechanically functional extracellular matrix" J Orthop Res 10(6): 745-58. PDF
|
| Book Chapters |
Michael D. Buschmann, Caroline D. Hoemann, Mark B. Hurtig, and Matthew S. Shive
Cartilage repair with chitosan-glycerol phosphate stabilized blood clots
In Cartilage Repair Strategies, Chapter 7, pp 85-104, 2007. |
| Shive MS , Hoemann CD , Restrepo A , Hurtig MB , Duval N , Ranger P , Stanish W , Buschmann MD. BST-CarGel: In Situ ChondroInduction for Cartilage Repair In Operative Techniques in Orthopaedics: Articular Cartilage Surgery. Ed Freddie Fu. 2006. - PDF (295KB) |
| Grodzinsky AJ, Kim YJ, Buschmann MD, Garcia AM, Quinn TM, Hunziker EB. Osteoarthritis 1998 |
| Grodzinsky AJ, Frank E, Kim YJ, Buschmann MD. Structure and Function of the Extracellular Matrix 1996 |
Gene Delivery |
|
The introduction into a cell of a DNA sequence encoding for a therapeutic protein can be used to stimulate tissue repair or to have therapeutic effects in cardiovascular, cancer and metabolic disease. Chitosan can be used as a carrier to take DNA into the cell and achieve production of the protein. This approach avoids the immunological and oncogenic problems associated with viral introduction of foreign DNA but suffers from a lower level of production of the protein compared to the viral approach. We have leveraged our expertise in chitosan production, characterization and polyeletrolyte behavior to create chitosan/DNA nanoparticles that achieve very high levels of transgene expression. We are studying the mechanisms responsible for these high transfection levels using both physicochemical characterization of chitosan-DNA interactions and the cell biological events involved in the transfection process. Animal studies are also underway to investigate the use of these gene-bearing nanoparticles as therapeutic agents. |
| |
| Scientific Articles |
Alameh M., DeJesus D., Jean M., Darras V., Thibault M., Lavertu M., Buschmann M. D. and Merzouki A. (2012). "Low molecular weight chitosan nanoparticulate system at low N:P ratio for nontoxic polynucleotide delivery" International Journal of Nanomedecine 7 1399-1414. PDF
| Jean M, Alameh M, De Jesus D, Thibault M, Lavertu M, Darras V, Nelea M, Buschmann MD, Merzouki A. (2012). "Chitosan-based therapeutic nanoparticles for combination gene therapy and gene silencing of in vitro cell lines relevant to type 2 diabetes" European Journal of Pharmaceutical Science 45(1-2): 138-149. PDF
| Jean M., Alameh M., Buschmann M. D. and Merzouki A. (2011). "Effective and safe gene-based delivery of GLP-1 using chitosan/plasmid-DNA therapeutic nanocomplexes in an animal model of type 2 diabetes" Gene Therapy 18(8): 807-816. PDF
| Thibault M., Astolfi M., Tran-Khanh N., Lavertu M., Darras V., Merzouki A. and Buschmann M. D. (2011). "Excess polycation mediates efficient chitosan-based gene transfer by promoting lysosomal release of the polyplexes" Biomaterials 32(Epub ahead of print): 4639-4646. PDF
| Nimesh S., Thibault M. M., Lavertu M. and Buschmann M. D. (2010). " Enhanced gene delivery mediated by low molecular weight chitosan/DNA complexes: effect of pH and serum" Molecular Biotechnology 46(2): 182-196. PDF
| Thibault M. M., S. Nimesh, M. Lavertu and MD. Buschmann (2010). " Intracellular trafficking and decondensation kinetics of Chitosan-pDNA polyplexes" Molecular therapy : the journal of the American Society of Gene Therapy 18(10): 1785-1795. PDF
| Alameh M., Jean M., DeJesus D., Buschmann M. D. and Merzouki A. (2010). "Chitosanase-based method for RNA isolation from cells transfected with chitosan/siRNA nanocomplexes for real-time RT-PCR in gene silencing" International Journal of Nanomedicine 5 473-481. PDF
| MacQueen L. A., Buschmann M. D. and Wertheimer M. R. (2010). " Mechanical properties of mammalian cells in suspension measured by electro-deformation" Journal of Micromechanics and Microengineering 20(6): 1-11. PDF
| Ma P. L., Buschmann M. D. and Winnik F. M. (2010). " Complete physicochemical characterization of DNA/Chitosan complexes by multiple detection using asymmetrical Flow Field-Flow Fractionation" Analytical Chemistry 82(23): 9636-9643. PDF
| Ma P. L., Lavertu M., Winnik F. M. and Buschmann M. D. (2009). " New insights into chitosan-DNA interactions using isothermal titration microcalorimetry" Biomacromolecules 10(6): 1490-1499. PDF
| Gigout A., Levasseur S., Girard-Lauriault P. L., Buschmann M. D., Wertheimer M. R. and Jolicoeur M. (2009). " CHO cells adhering to nitrogen-rich plasma-polymerised ethylene exhibit high production of a specific recombinant protein" Macromolecular Bioscience 9(10): 979-988. PDF
| Jean M., Smaoui F., Lavertu M., Methot S., Bouhdoud L., Buschmann M. D. and Merzouki A. (2009). " Chitosan-plasmid nanoparticle formulations for IM and SC delivery of recombinant FGF-2 and PDGF-BB or generation of antibodies" Gene Therapy 16(9): 1097-1110. PDF
| MacQueen L. A., Buschmann M. D. and Wertheimer M. R. (2008). " Gene delivery by electroporation after dielectrophoretic positioning of cells in a non-uniform electric field" Bioelectrochemistry 72(2): 141-148. PDF
| Lavertu M., Filion D. and Buschmann M. D. (2008). " Heat-induced transfer of protons from chitosan to glycerol phosphate produces chitosan precipitation and gelation" Biomacromolecules 9(2): 640-650. PDF
| Filion D., Lavertu M. and Buschmann M.D. (2007). " Ionization and solubility of chitosan solutions related to thermosensitive chitosan/glycerol-phosphate systems" Biomacromolecules 8(NA): 3224-3234. PDF
| Lavertu M., Methot S., Tran-Khanh N. and Buschmann M. D. (2006). " High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation" Biomaterials 27(27): 4815-24. PDF
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Biomechanics |
|
The ultimate function of joint tissues such as cartilage, meniscus and bone is biomechanical. Articular cartilage, for example, must withstand and survive enormous pressures that can exceed 100 times atmospheric pressure and simultaneously provide joints surfaces that have almost no friction. The molecular structure and physical mechanisms that endow articular cartilage with these abilities are only partly elucidated. We develop new instruments that test the biomechanical properties of cartilage and its interesting electrical behavior where electric fields are produced inside cartilage when it is compressed. Mathematical models are also used to accurately predict the forces developed in cartilage under different loading conditions. These models account for the presence of a solid cross-linked macromolecular network and the pressurized fluid within it. The generation of electric fields in compressed cartilage is also measured using systems of microelectrodes. This latter technology has been translated to industry in the design of an instrument ( http://www.biomomentum.com/Arthro-BST.html) that is being used to map the function of articular cartilage in patients undergoing arthroscopic surgery. |
| |
| Scientific Articles |
Amini, S.,
Mortazavi, F.,
Sun, J.,
Levesque, M.,
Hoemann, C. D.,
Villemure, I. (2013). "Stress relaxation of swine growth plate in semi-confined compression: depth dependent tissue deformational behavior versus extracellular matrix composition and collagen fiber organization" Biomechanics and Modeling in Mechanobiology 12(1): 67-68. PDF
| Hoemann C. D.,
Lafantaisie-Favreau C. H.,
Lascau-Coman V.,
Chen G.
Guzmán-Morales J. (2012). "The cartilage-bone interface" The Journal of Knee Surgery 25(2): 85-97. PDF
| Kazemi M., Li L. P., Buschman M. D. and Savard P. (2011). "Partial meniscetomy changes fluid pressurization in articular cartilage in human knees" Journal of Biomechanical Engineering In press . PDF
| Kazemi M., Li L. P., Savard P. and Buschmann M. D. (2011). "Creep behavior of the intact and meniscectomy knee joints" Journal of the Mechanical Behavior of Biomedical Materials 4(7): 1351-1358. PDF
| Changoor A., Coutu J. P., Garon M., Quenneville E., Hurtig M. B. and Buschman M. D. (2011). "Streaming potential-based arthroscopic device is sensitive to cartilage changes immediately post-impact in an equine cartilage injury model" Journal of Biomechanical Engineering-Transactions of the Asme 133(6): 1-10. PDF
| Changoor A., Fereydoonzad L., Yaroshinsky A. and Buschmann M. D. (2010). " Effects of refrigeration and freezing on the electromechanical and biomechanical properties of articular cartilage" Journal of Biomechanical Engineering-Transactions of the Asme 132(6): 1-6. PDF
| Valverde-Franco G., Binette J. S., Li W., Wang H., Chai S., Laflamme F., Tran-Khanh N., Quenneville E., Meijers T., Poole A. R., Mort J. S., Buschmann M. D. and Henderson J. E. (2006). " Defects in articular cartilage metabolism and early arthritis in fibroblast growth factor receptor 3 deficient mice" Hum Mol Genet 15(11): 1783-92. PDF
| Tran-Khanh N., Hoemann C. D., McKee M. D., Henderson J. E. and Buschmann M. D. (2005). " Aged bovine chondrocytes display a diminished capacity to produce a collagen-rich, mechanically functional cartilage extracellular matrix" J Orthop Res 23(6): 1354-62. PDF
| Quenneville Eric and Buschmann Michael D. (2005). " A transport model of electrolyte convection through a charged membrane predicts generation of net charge at membrane/electrolyte inerfaces" Journal of Membrane Science 265(NA): 60-73. PDF
| Hoemann C. D., Sun J., Legare A., McKee M. D. and Buschmann M. D. (2005). " Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell-delivery vehicle" Osteoarthritis Cartilage 13(4): 318-329. PDF
| Charlebois M., McKee M. D. and Buschmann M. D. (2004). "Nonlinear tensile properties of bovine articular cartilage and their variation with age and depth" Journal of Biomechanical Engineering 126(NA): 129-137. PDF
| Binette J. S., Garon M., Savard P., McKee M. D. and Buschmann M. D. (2004). " Tetrapolar measurement of electrical conductivity and thickness of articular cartilage" Journal of Biomechanical Engineering 126(4): 475-484. PDF
| Quenneville E., Binette J. S., Garon M., Legare A., Meunier M. and Buschmann M. D. (2004). " Fabrication and characterization of nonplanar microelectrode array circuits for use in arthroscopic diagnosis of cartilage diseases" IEEE Transactions on Biomedical Engineering 51(12): 2164-2173. PDF
| Gauthier M. A., Luo J., Calvet D., Ni C., Zhu X. X., Garon M. and Buschmann M. D. (2004). " Degree of crosslinking and mechanical properties of crosslinked poly(vinyl alcohol) beads for use in solid-phase organic synthesis" Polymer 45(NA): 8201-8210. PDF
| Li L. P., Buschmann M. D. and Shirazi-Adl A. (2003). " Strain-rate dependent stiffness of articular cartilage in unconfined compression" J Biomech Eng 125(2): 161-168. PDF
| Jurvelin J. S., Buschmann M. D. and Hunziker E. B. (2003). " Mechanical anisotropy of the human knee articular cartilage in compression" Proc Inst Mech Eng [H] 217(3): 215-219. PDF
| Fortin M., Buschmann M. D., Bertrand M. J., Foster F. S. and Ophir J. (2003). " Dynamic measurement of internal solid displacement in articular cartilage using ultrasound backscatter" Journal of Biomechanics 36(3): 443-447. PDF
| Li L. P., Shirazi-Adl A. and Buschmann M. D. (2003). " Investigation of mechanical behavior of articular cartilage by fibril reinforced poroelastic models" Biorheology 40(NA): 227-233. PDF
| Thibault M., Poole A. R. and Buschmann M. D. (2002). " Cyclic compression of cartilage/bone explants in vitro leads to physical weakening, mechanical breakdown of collagen and release of matrix fragments" Journal of Orthopaedic Research 20(6): 1265-1273. PDF
| Ravenelle F., Marchessault R. H., Legare A. and Buschmann M. D. (2002). " Mechanical properties and structure of swollen crosslinked high amylose starch tablets" Carbohydrate Polymers 47(3): 259-266. PDF
| Li L. P., Buschmann M. D. and Shirazi-Adl A. (2002). " The role of fibril reinforcement in the mechanical behavior of cartilage" Biorheology 39(NA): 89-96. PDF
| Langelier Eve and Buschmann Michael D. (2002). " Increasing strain and strain-rate strengthens transient stiffness but weakens the response to subsequent compression for articular cartilage in unconfined compression" Journal of Biomechanics 36(6): 853-859. PDF
| Li L., Shirazi-Adl A. and Buschmann M. (2002). " Alterations in mechanical behavior of articular cartilage due to changes in depth varying material properties a nonhomogeneous poroelastic model study" Computer Methods in Biomechanics and Biomedical Engineering 5(1): 45-52. PDF
| Garon M., Légaré A., Guardo R., Savard P. and Buschmann M. D. (2002). " Streaming potentials maps are spatially resolved indicators of amplitude, frequency and ionic strenght dependant responses of articular cartilage to load" Journal of Biomechanics 35(2): 207-216. PDF
| Hoemann C. D., Sun J., Chrzanowski V. and Buschmann M. D. (2002). " A multivalent assay to detect glycosaminoglycan, protein, collagen, RNA, and DNA content in milligram samples of cartilage or hydrogel- based repair cartilage" Anal Biochem 300(1): 1-10. PDF
| Légaré A., Garon M., Guardo R., Savard P., Poole A. R. and Buschmann M. D. (2002). " Detection and analysis of cartilage degeneration by spatially resolved streaming potentials in articular cartilage" Journal of Orthopaedic Research 20(4): 819-826. PDF
| Li L. P., Buschmann M. D. and Shirazi-Adl A. (2001). " The asymmetry of transient response in compression vs release for cartilage in unconfined compression" Journal of Biomechanics 123(NA): 519-522. PDF
| Joiner G. A., Bogoch E. R., Pritzker K. P., Buschmann M. D., Chevrier A. and Foster F. S. (2001). " High frequency acoustic parameters of human and bovine articular cartilage following experimentally-induced matrix degradation" Ultrason Imaging 23(2): 106-16. PDF
| Langelier E., Suetterlin R., Hoemann C. D., Aebi U. and Buschmann M. D. (2000). " The chondrocyte cytoskeleton in mature articular cartilage: structure and distribution of actin, tubulin, and vimentin filaments" J Histochem Cytochem 48(10): 1307-1320. PDF
| Fortin M., Soulhat J., Shirazi-Adl A., Hunziker E. B. and Buschmann M. D. (2000). " Unconfined compression of articular cartilage: nonlinear behavior and comparison with a fibril-reinforced biphasic model" Journal of Biomechanical Engineering 122(NA): 189-195. PDF
| Chenite A., Chaput C., Wang D., Combes C., Buschmann M. D., Hoemann C. D., Leroux J. C., Binette F. and Selmani A. (2000). " Novel injectable neutral solutions of chitosan form biodegradable gels in situ" Biomaterials 21(21): 2155-2161. PDF
| Li L. P., Buschmann M. D. and Shirazi-Adl A. (2000). " A fibril reinforced nonhomogeneous poroelastic model for articular cartilage: inhomogeneous response in unconfined compression" Journal of Biomechanics 33(12): 1533-1541. PDF
| Buschmann M. D., Maurer A. M., Berger E. and Hunziker E. B. (2000). "Ruthenium hexaammine trichloride chemography for aggrecan mapping in cartilage is a sensitive indicator of matrix degradation" The Journal of Histochemistry & Cytochemistry 48(1): 81-88. PDF
| Li L. P., Soulhat J., Buschmann M. D. and Shirazi-Adl A. (1999). " Nonlinear analysis of cartilage in unconfined ramp compression using a fibril reinforced poroelastic model" Clinical Biomechanics 14(9): 673-682. PDF
| Buschmann M. D., Kim Y. J., Wong M., Frank E., Hunziker E. B. and Grodzinsky A. J. (1999). " Stimulation of aggrecan synthesis in cartilage explants by cyclic loading is localized to regions of high interstitial fluid flow" Archives of Biochemistry and Biophysics 366(1): 1-7. PDF
| Soulhat J., Buschmann M. D. and Shirazi-Adl A. (1999). " A fibril-network reinforced biphasic model of cartilage in unconfined compression" Journal of Biomechanical Engineering 121(3): 340-347. PDF
| Dumont J., Ionescu M., Reiner A., Poole A. Robin, Tran-Khanh N., Hoemann C. D., McKee M. and Buschmann M. D. (1999). " Mature full-thickness articular cartilage explants attached to bone are physiologically stable over long-term culture in serum-free media" Connective Tissue Research 40(4): 259-272. PDF
| Buschmann M. D., Soulhat J., Shirazi-Adl A., Jurvelin J. S. and Hunziker E. B. (1998). " Confined compression of articular cartilage: linearity in ramp and sinusoidal tests and the importance of interdigitation and incomplete confinement" Journal of Biomechanics 31(2): 171-178. PDF
| Quinn T. M., Grodzinsky A. J., Buschmann M. D., Kim Y. J. and Hunziker E. B. (1998). " Mechanical compression alters proteoglycan deposition and matrix deformation around individual cells in cartilage explants" J Cell Sci 111(NA): 573-583. PDF
| Jurvelin J. S., Buschmann M. D. and Hunziker E. B. (1997). " Optical and mechanical determination of Poisson's ratio of adult bovine humeral articular cartilage" Journal of Biomechanics 30(3): 235-41. PDF
| Buschmann M. D. (1997). " Numerical conversion of transient to harmonic response functions for linear viscoelastic materials" Journal of Biomechanics 30(2): 197-202. PDF
| Wong M., Wuethrich P., Buschmann M. D., Eggli P. and Hunziker E. (1997). " Chondrocyte biosynthesis correlates with local tissue strain in statically compressed adult articular cartilage" Journal of Orthopaedic Research 15(2): 189-96. PDF
| Buschmann M. D., Hunziker E. B., Kim Y. J. and Grodzinsky A. J. (1996). " Altered aggrecan synthesis correlates with cell and nucleus structure in statically compressed cartilage" Journal of Cell Science 109(NA): 499-508. PDF
| Buschmann M. D. and Grodzinsky A. J. (1995). " A molecular model of proteoglycan-associated electrostatic forces in cartilage mechanics " Journal of Biomechanical Engineering-Transactions of the Asme 117(2): 179-192. PDF
| Buschmann M. D., Gluzband Y. A., Grodzinsky A. J. and Hunziker E. B. (1995). " Mechanical compression modulates matrix biosynthesis in chondrocyte agarose cultures" Journal of Cell Science 108(NA): 1497-1508. PDF
| Brink H. F., Buschmann M. D. and Rosen B. R. (1989). " NMR chemical shift imaging" Computerized Medical Imaging and Graphics 13(1): 93-104. PDF
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